The EZBioscience^® Universal RNA Purification Kit integrates phenol/guanidine-based sample lysis and silica-membrane purification of high-quality total RNA from animal tissues and cells. Samples are first lysed and homogenized in Lysis Buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After addition of Buffer A, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase while DNA partitions to the interphase and proteins to the lower, organic phase or the interphase. The upper, aqueous phase is collected, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the RNA Spin Column, where the total RNA binds to the membrane, and contaminants are effectively washed away. Finally, high-quality RNA is eluted in Elution Buffer. The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, RNA-seq, Northern blotting, Poly A+ RNA selection, Microarray analysis, and so on.

Components

*Before using for the first time, add 52 ml of ethanol to the Wash Buffer 1, 52 ml of ethanol to the Wash Buffer 2.

Storage

Store the Lysis Buffer and Buffer A at 2 ~ 8°C, protect from light. Store other components at room temperature (When using these buffers, be careful to avoid of contamination). Divide the Elution Buffer into small aliquots upon reception is suggested