The EZBioscience^® EZ-press RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of sources, without the need for toxic substances such as phenol or chloroform. The Kit can be used with cultured cells. Biological samples are first lysed and homogenized in a strong denaturant containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, ethanol is added to the lysate, creating conditions that promote selective binding of RNA to the silica-based membrane. The sample is then applied to the Spin Column, where the total RNA binds to the membrane, then the membrane is treated with gDNA Remover to eliminate the DNA. Contaminants are efficiently washed away by subsequent step of washing. The high-quality total RNA is then eluted in Elution Buffer. The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, and so on.

Components

Components	B0004DP (100 Preps)
Lysis Buffer	55 ml
Wash Buffer*	13 ml
Elution Buffer	25 ml
gDNA Remover	220 μl
Spin Columns (with Collection Tubes)	100 Preps

*Before using for the first time, add 52 ml ethanol to the Wash Buffer and mix thoroughly.   

Storage

Store the gDNA Remover at -20°C. Store other components at room temperature (When using these buffers, be careful to avoid of contamination).