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Clone Name
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UMAB253
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Applications
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10k-ChIP, IF, IHC, WB
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Recommend Dilution
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WB 1:2000, IHC 1:100~300, IF 1:100
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Reactivity
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Human, Mouse, Rat
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Host
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Mouse
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Clonality
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Monoclonal
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Immunogen
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Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 (NP_005068) produced in E.coli.
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Formulation
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Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
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Reconstitution Method
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For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
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Purification
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Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
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Conjugation
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Unconjugated
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Storage Condition
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Store at -20°C as received.
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Predicted Protein Size
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83 kDa
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Gene Name
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Homo sapiens TLE family member 1, transcriptional corepressor (TLE1), transcript variant 2, mRNA.
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Synonyms
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ESG; ESG1; GRG1
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