- 品牌:Polyplus
- 产地:中国
- 型号:0.4 mL
- 货号:101000010
- 价格: ¥20/盒
- 发布日期: 2024-03-05
- 更新日期: 2024-08-12
产地 | 中国 |
保存条件 | -20℃ |
品牌 | Polyplus |
货号 | 101000010 |
用途 | 详询 |
组织来源 | 详询 |
细胞形态 | 详询 |
是否是肿瘤细胞 | 否 |
保质期 | 一年 |
器官来源 | 详询 |
免疫类型 | 详询 |
品系 | 详询 |
生长状态 | 详询 |
物种来源 | 详询 |
包装规格 | 0.4 mL |
是否进口 | 否 |
Specifications
Reagent |
PULSin® |
---|---|
Molecule delivered |
Protein; Antibody; Peptide |
Cell types |
Adherent & suspension cells grown in presence of serum |
Number of transfection |
0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates |
Storage |
Store PULSin® at 5 °C ± 3°C. Do not freeze. |
Provided with |
Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control |
Overview
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.
PULSin®?is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.
Ordering Information
Reference number | Amount of reagent | Positive control (R-phycoerythrin) | Hepes dilution buffer |
---|---|---|---|
101000010 | PULSin® 0.4 mL | 20 μg | 20 ml |