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Polyplus蛋白质、抗体&多肽转染试剂PULSin®(101000010)
  • 品牌:Polyplus
  • 产地:中国
  • 型号:0.4 mL
  • 货号:101000010
  • 价格: ¥20/盒
  • 发布日期: 2024-03-05
  • 更新日期: 2024-08-12
产品详请
产地 中国
保存条件 -20℃
品牌 Polyplus
货号 101000010
用途 详询
组织来源 详询
细胞形态 详询
是否是肿瘤细胞
保质期 一年
器官来源 详询
免疫类型 详询
品系 详询
生长状态 详询
物种来源 详询
包装规格 0.4 mL
是否进口

Specifications

Reagent

PULSin®

Molecule delivered

Protein; Antibody; Peptide

Cell types

Adherent & suspension cells grown in presence of serum

Number of transfection

0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates

Storage

Store PULSin® at 5 °C ± 3°C. Do not freeze.
Expiry date is indicated in the certificate of analysis and on the product.

Provided with

Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control


Overview

The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.

PULSin®?is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.


Ordering Information

Reference number Amount of reagent Positive control (R-phycoerythrin) Hepes dilution buffer
101000010 PULSin® 0.4 mL 20 μg 20 ml