Product Introduction:Aldehyde-activated agarose beads have a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to aldehyde-activated agarose is a successful and well-documented technique. Aldehyde-activated SepFast media forms chemically stable secondary amine linkages with ligands containing primary amine groups through reductive amination reaction. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

Product Properties:

There is a choice of 2 different base matrices that suit various molecules to be coupled or purified. ?

• Aldehyde-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. Its large pore size is suitable for coupling or purifying large molecules. It also shows high mechanical rigidity allowing high flow throughput with reduced back pressure. 

• Aldehyde-activated SepFast 6 High Flow (6HF) is made of highly cross-linked 6% beaded agarose. Its moderate pore size is suitable for coupling smaller molecules. It also shows high mechanical rigidity allowing high flow throughput with reduced back pressure.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules. The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents. Aldehyde-activated SepFast media is supplied as an aqueous suspension in 20% ethanol.

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