Product Introduction:

NHS (N-hydroxysuccinimide)-activated SepFast MAG is used to make magnetic adsorbents coupled with molecules containing amine groups. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

NHS-activated SepFast MAG media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides.

This pre-activated resin can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

Product Properties:

NHS activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with Nhydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFasm MAG media is supplied as a suspension in isopropanol.

Product Series: