北京百奥创新科技有限公司
全国咨询热线:15522676233
NHS活化琼脂糖凝胶4HF
  • 品牌:Biotoolomics
  • 产地:英国
  • 货号:330101-5ML
  • 发布日期: 2023-04-10
  • 更新日期: 2024-08-12
产品详请
品牌 Biotoolomics
货号 330101-5ML
用途 层析纯化
是否危险化学品
包装规格 5mL;50mL;1L
别名 NHS活化琼脂糖层析介质
CAS编号
产地/厂商 英国
是否进口

NHS活化琼脂糖层析填料

Product Introduction:

NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.

NHS-activated SepFast media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

Product Properties:

NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with N-hydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFast media is supplied as a suspension in isopropanol.

Product Series:

Product Quantity Code no.
NHS-activated SepFast 4HF 5 ml 330101-5ML

50 ml 330101-50ML

1 Litre 330101-1L