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不可逆硫醇耦联磁性琼脂糖凝胶6HF
  • 品牌:Biotoolomics
  • 产地:英国
  • 货号:390204-5ML
  • 发布日期: 2023-04-07
  • 更新日期: 2024-08-12
产品详请
品牌 Biotoolomics
货号 390204-5ML
用途 层析纯化
是否危险化学品
包装规格 5mL;50mL;1L
别名 不可逆硫醇耦联磁性琼脂糖介质
CAS编号
产地/厂商 英国
是否进口

不可逆硫醇耦联磁性琼脂糖层析填料

Product Introduction:

Irreversible Thiol-coupling SepFast MAG is used to make magnetic adsorbents to immobilize thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or special equipment is required.

Irreversible Thiol-coupling SepFast MAG medium forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm (12 atoms) that facilitates much improved ligand accessibility. This pre-activated magnetic agarose resin can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.

Product Properties:

There is a choice of two different activated base matrices that suit ligands of various molecular weights.

  • Irreversible Thiol-Coupling SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.

  • Irreversible Thiol-Coupling SepFast MAG 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity and allows harsher operational conditions.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm (12 atoms). It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.

Group to be coupled -SH
Matrix

SepFast MAG 4HF: highly cross-linked 4% magnetic agarose

SepFast MAG 6HF: highly cross-linked 6% magnetic agarose

Particle size* 50 – 150 μm
Coupling level At least 5 mg BSA / ml medium
pH stability 4 -11 (ligand dependent)
Chemical stability

Compatible with all commonly used aqueous chemicals, provided the ligand to be

coupled can withstand

Storage +4℃ - +8℃

Product Series:

Product Quantity   Code no.
Irreversible Thiol-coupling SepFast MAG 4HF 5 ml 390201-5ML

50 ml 390201-50ML

1 Litre 390201-1L
Irreversible Thiol-coupling SepFast MAG 6HF 5 ml 390204-5ML

50 ml 390204-50ML

1 Litre 390204-1L