不可逆硫醇耦联琼脂糖层析填料

Product Introduction:

Irreversible Thiol-coupling SepFast can be used for the specific immobilization of thiol containing molecules. The coupling chemistry is based on a haloacetyl activation method that is a successful and well-documented technique. The reaction to the thiol group is highly specific and the formed bond is irreversible. The coupling reaction is rapid and easy to carry out. No toxic chemicals or equipment is required. 

Irreversible Thiol-coupling SepFast media forms an extremely stable thioether bond with ligands containing thiol (i.e. sulphhydryl) groups. It has a long spacer arm that facilitates much improved ligand accessibility. This pre-activated agarose based resin can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

Product Properties:

There is a choice of two different activated base matrices that suit ligands of various molecular weights.

• Irreversible Thiol-Coupling SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.

• Irreversible Thiol-Coupling SepFast 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The base matrix is activated by attaching a haloacetyl group through a long hydrophilic spacer arm. It reacts rapidly with molecules containing thiol groups. Irreversible Thiol-coupling SepFast media is supplied as an aqueous suspension.

Group to be coupled-SH

Matrix：

Particle size：50 – 150 μm

Coupling level：At least 3 mg BSA / ml medium

pH stability3 -11 (ligand dependent)

Chemical stabilityCompatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand

Storage+4℃ - +8℃

Product Series: