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环氧活化磁性琼脂糖凝胶4HF
  • 品牌:Biotoolomics
  • 产地:英国
  • 货号:410201-5ML
  • 发布日期: 2023-04-06
  • 更新日期: 2024-08-12
产品详请
品牌 Biotoolomics
货号 410201-5ML
用途 层析纯化
是否危险化学品
包装规格 5mL;50mL;1L
别名 环氧活化磁性层析填料
CAS编号
产地/厂商 英国
是否进口

环氧树脂活化磁性琼脂糖层析填料

Product Introduction:

Epoxy-activated SepFast MAG is a magnetic agarose resin that can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications. 

Epoxy-activated agarose can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. It can also be used for the coupling of primary aminecontaining or thiol-containing molecules. Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemical or special equipment is required. All the chemical bonds formed are very stable. 

Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various magnetic affinity chromatography media for both small scale and large scale purification applications.

Product Properties:

There is a choice of two different Epoxy activated magnetic resins that suits various ligands and applications. 

   ? Epoxy activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied. It is useful for the coupling of protein molecules.  

   ? Epoxy activated SepFast MAG 6HF is made of highly cross-linked 6% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions, such as concentrated organic solvents, to be applied. It has high levels of epoxy groups for applications that require high ligand densities. It is useful for the coupling of small molecules or peptides etc. 

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules. The material is activated through attaching a hydrophilic carbon chain with epoxy group in the end.

Group to be coupled -NH2, -OH, -SH
Matrix

SepFast MAG 4HF: Highly cross-linked 4% magnetic agarose

 SepFast MAG 6HF: Highly cross-linked 6% magnetic agarose

Particle size* 50 – 150 μm
Activation level

>30 μmol epoxy / ml for SepFast MAG 4HF

>80 μmol epoxy / ml for SepFast MAG 6HF

pH stability 4 -13 (ligand dependent)
Chemical stability

Compatible with all commonly used aqueous chemicals, provided the ligand to

 be coupled can withstand. Certain organic solvents could be used.

Storage +4℃ - +8℃

Product Series:

Product Quantity Code no.
Epoxy activated SepFast MAG 4HF 5 ml 410201-5ML

50 ml 410201-50ML

1 litre 410201-1L
Epoxy activated SepFast MAG 6HF 5 ml 410204-5ML

50 ml 410204-50ML

1 litre 410204-1L