北京百奥创新科技有限公司
全国咨询热线:15522676233
环氧活化琼脂糖凝胶6HF
  • 品牌:Biotoolomics
  • 产地:英国
  • 货号:410104-50mL
  • 发布日期: 2023-04-06
  • 更新日期: 2024-08-12
产品详请
品牌 Biotoolomics
货号 410104-50mL
用途 层析纯化
是否危险化学品
包装规格 50mL;300mL;1L
别名 环氧活化琼脂糖填料
CAS编号
产地/厂商 英国
是否进口

环氧树脂活化琼脂糖层析填料

Product Introduction:

Epoxy-activated agarose beads can be used for the immobilization of sugars and other carbohydrates via stable ether linkages with hydroxyl groups. They can also be used for the coupling of primary amine-containing, or thiol-containing molecules.

Coupling biospecific ligands to Epoxy-activated agarose is a successful and well-documented technique. The reaction is very easy to conduct. No toxic chemical or special equipment is required. All the chemical bonds formed are very stable.

Epoxy activated SepFast is useful in coupling both small molecules and very large molecules. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography media for both small scale and large scale purification applications.

Product Properties:

There is a choice of two different Epoxy activated base matrices that suits various ligands and applications.

  • Epoxy activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows good mechanical rigidity allowing high flow throughput with reduced back pressure. It is useful for the coupling of protein molecules.

  • Epoxy activated SepFast 6HF is made of highly cross-linked 6% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure. It has high levels of epoxy groups for applications that require high ligand densities and high flow properties. It is useful for the coupling of small molecules or peptides etc.

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.

The material is activated through attaching a hydrophilic carbon chain with epoxy group in the end.

Group to be coupled

-NH2, -OH, -SH

Matrix

SepFast 4HF: Highly cross-linked 4% agarose bead

SepFast 6HF: Highly cross-linked 6% agarose bead

Particle size

50 – 150 μm

Activation level

> 30 μmol epoxy / ml for SepFast 4HF

>80 μmol epoxy / ml for SepFast 6HF

pH stability

2 -13 (ligand dependent)

Chemical stability

Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand. Certain organic solvents could be used.

Storage

+4℃—+8℃

Product Series:

Product Quantity Code no.
Epoxy activated SepFast 4HF 50 ml 410101-50ML

300 ml 410101-300ML

1 litre 410101-1L
Epoxy activated SepFast 6HF 50 ml 410104-50ML

300 ml 410104-300ML

1 litre 410104-1L