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胺基活化琼脂糖凝胶6HF
  • 品牌:Biotoolomics
  • 产地:英国
  • 货号:350301-5ML
  • 发布日期: 2023-04-04
  • 更新日期: 2024-08-12
产品详请
品牌 Biotoolomics
货号 350301-5ML
用途 层析纯化
是否危险化学品
包装规格 5mL;50mL;1L
别名 胺基活化层析介质
CAS编号
产地/厂商 英国
是否进口

胺基活化琼脂糖层析填料

Product Introduction:Amine-activated agarose beads can be used for the immobilization of carboxyl containing or aldehyde containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique. Amine-activated SepFast forms a chemically stable amide linkage with ligands containing carboxyl groups. It has a long spacer (9 atoms). The pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.

胺基特性.png

Product Properties:

Amine-activated SepFast 4 High Flow (4HF) is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure. 

Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. 

The base matrix is activated by attaching a long hydrophilic chain with an amine group in the end. They react with either carboxyl containing ligands in the presence of a carbodiimide agent, or aldehyde containing ligands in the presence of a reducing agent. Amine-activated SepFast is supplied as an aqueous suspension in 20% ethanol.

Group to be coupled

-COOH, CHO

Matrix

Highly cross-linked 4% agarose beads (4HF)

Particle size

50 – 150 μm

Particle size

50 – 150 μm

Spacer arm

9 atoms

Activation level

10 - 25 μmol amine / ml medium

pH stability

2 -13 (ligand dependent)

Chemical stability

Compatible with all commonly used aqueous chemicals, provided the ligand to be coupled can withstand

Storage

+4℃—+8

Product Series:

Product

                  Quantity

 Code no.

Amine-activated SepFast 4HF

                  5 ml                     

350101-5ML


                  50 ml

350101-50ML


                  1 Litre

350101-1L

Amine-activated SepFast 6HF

                  5 ml

350301-5ML


                  50 ml

350301-50ML


                  1 Litre

350301-1L