- 品牌:Invivogen
- 产地:国外
- 型号:详询
- 货号:bb-kostg
- 发布日期: 2023-03-31
- 更新日期: 2024-08-12
产地 | 国外 |
保存条件 | 详询 |
品牌 | Invivogen |
货号 | bb-kostg |
用途 | 详询 |
组织来源 | 详询 |
细胞形态 | 详询 |
是否是肿瘤细胞 | 是 |
保质期 | 详询 |
器官来源 | 详询 |
免疫类型 | 详询 |
品系 | 详询 |
生长状态 | 详询 |
物种来源 | 详询 |
包装规格 | 详询 |
是否进口 | 是 |
B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway.
B16-Blue™ ISG-KO-STING cells were generated from the B16-Blue™ ISG cell line, a murine B16- F1 melanoma-derived cell line, through stable knockout of the STING gene. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
B16-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, DMXAA, canonical and non-canonical CDNs while retaining the ability to respond to type I and type II IFNs.
Stimulation of these cells with IFN triggers the activation of the I-ISG54 promoter and the production of SEAP. Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.