北京百奥创新科技有限公司
全国咨询热线:15522676233
B16-Blue™ ISG-KO-STING cells can be used for the study of th
  • 品牌:Invivogen
  • 产地:国外
  • 型号:详询
  • 货号:bb-kostg
  • 发布日期: 2023-03-31
  • 更新日期: 2024-08-12
产品详请
产地 国外
保存条件 详询
品牌 Invivogen
货号 bb-kostg
用途 详询
组织来源 详询
细胞形态 详询
是否是肿瘤细胞
保质期 详询
器官来源 详询
免疫类型 详询
品系 详询
生长状态 详询
物种来源 详询
包装规格 详询
是否进口

B16-Blue™ ISG-KO-STING cells can be used for the study of the STING signaling pathway and for the detection of bioactive murine types I and II IFNs by monitoring the activation of the JAK/STAT pathway.

B16-Blue™ ISG-KO-STING cells were generated from the B16-Blue™ ISG cell line, a murine B16- F1 melanoma-derived cell line, through stable knockout of the STING gene. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

B16-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, DMXAA, canonical and non-canonical CDNs while retaining the ability to respond to type I and type II IFNs.

Stimulation of these cells with IFN triggers the activation of the I-ISG54 promoter and the production of SEAP. Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.