- 品牌:Invivogen
- 产地:国外
- 型号:详询
- 货号:hkb-kotifa
- 发布日期: 2023-03-31
- 更新日期: 2024-08-12
产地 | 国外 |
保存条件 | -20℃保存 |
品牌 | Invivogen |
货号 | hkb-kotifa |
用途 | 科研 |
组织来源 | 详询 |
细胞形态 | 详询 |
是否是肿瘤细胞 | 是 |
保质期 | 详询 |
器官来源 | 详询 |
免疫类型 | 详询 |
品系 | 详询 |
生长状态 | 详询 |
物种来源 | 详询 |
包装规格 | 详询 |
是否进口 | 是 |
Cell Line Stability
Cells will undergo genotypic changes over time resulting in reduced responsiveness in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably transfected cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages. HEK-Blue™ KO-TIFA cells should not be passaged more than 20 times to remain fully functional.
Quality Control
? TIFA gene knockout has been verified by DNA sequencing, RT-qPCR, and functional assays.
? The stability for 20 passages following thawing has been verified.
? These cells are guaranteed mycoplasma-free.
CELL LINE DESCRIPTION
HEK-Blue™ KO-TIFA cells were generated from HEK-Blue™ Null1-v cells through the stable knockout of the TIFA gene. The parental cells derive from human embryonic kidney 293 (HEK-293) cells. HEK-Blue™ KO-TIFA cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of an NF-κB-inducible promoter comprised of an IFN-β minimal promoter fused to five NF-κB and AP-1 binding sites. Levels of SEAP in the supernatant can be easily determined with HEK-Blue™ Detection, a SEAP detection cell culture medium. Unlike their parental cell line, HEK?Blue™ KO-TIFA cells do not respond to cytosolic ADP?Heptose. However, they do respond to other NF-κB inducers such as TNF-α and IL-1β. HEK?Blue™ KO-TIFA cells are resistant to Zeocin™