- 品牌:Invivogen
- 产地:国外
- 型号:详询
- 货号:hctd-nfis
- 发布日期: 2023-03-31
- 更新日期: 2024-08-12
产地 | 国外 |
保存条件 | -20℃保存 |
品牌 | Invivogen |
货号 | hctd-nfis |
用途 | 科研 |
组织来源 | 详询 |
细胞形态 | 详询 |
是否是肿瘤细胞 | 是 |
保质期 | 详询 |
器官来源 | 详询 |
免疫类型 | 详询 |
品系 | 详询 |
生长状态 | 详询 |
物种来源 | 详询 |
包装规格 | 详询 |
是否进口 | 是 |
HCT116-Dual™ cells are adherent epithelial cells that have been derived from the human HCT116 colorectal carcinoma cell line by stable integration of two inducible reporter constructs.
HCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP--1 binding sites.
HCT116-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.
As a result, HCT116-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase.
Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.