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Human NF-κB-SEAP & IRF-Luc Reporter colorectal carcinoma
  • 品牌:Invivogen
  • 产地:国外
  • 型号:详询
  • 货号:hctd-nfis
  • 发布日期: 2023-03-31
  • 更新日期: 2024-08-12
产品详请
产地 国外
保存条件 -20℃保存
品牌 Invivogen
货号 hctd-nfis
用途 科研
组织来源 详询
细胞形态 详询
是否是肿瘤细胞
保质期 详询
器官来源 详询
免疫类型 详询
品系 详询
生长状态 详询
物种来源 详询
包装规格 详询
是否进口

HCT116-Dual™ cells are adherent epithelial cells that have been derived from the human HCT116 colorectal carcinoma cell line by stable integration of two inducible reporter constructs.

HCT116-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP--1 binding sites.

HCT116-Dual™ cells also express the Lucia luciferase gene, which encodes a secreted luciferase, under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements.

As a result, HCT116-Dual™ cells allow to simultaneously study the NF-κB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase.

Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI-Blue™ Solution, a SEAP detection reagent, and QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.