Cell line description:

B16-Blue™ IFN-α/β cells derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP (secreted embryonic alkaline phosphatase) reporter gene under the control of the IFN-α/β-inducible ISG54 promoter enhanced by a multimeric ISRE. These cells do not respond to IFN-γ, due to the inactivation of the IFN-γ receptor.

B16-Blue™ IFN-α/β cells respond specifically to mIFN-α/β and do not respond to human IFN-α/β. Stimulation of these cells with murine IFN-α or IFN-β, or type I IFN inducers, such as poly(I:C), poly(dA:dT) or 5’ppp-dsRNA delivered intracellularly, triggers the production of SEAP  by the activation of the IRF-inducible promoter. Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™ Solution.