Contents and Storage

? 3-7 x 106 A549-hACE2-TMPRSS2 cells in a cryovial or shipping flask IMPORTANT: If cells provided in a cryovial are not frozen upon arrival, contact InvivoGen immediately.

? 1 ml of Puromycin (10 mg/ml), store at 4°C or at -20°C.*

? 1 ml of HygromycinB Gold (100 mg/ml), store at 4°C or at -20°C.*

? 1 ml of Normocin™ (50 mg/ml): a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. Store at -20°C.*

*The expiry date is specified on the product label. Note: Data sheets for all components are available on our website. Handling Frozen Cells Upon Arrival Cells must be thawed immediately upon receipt and grown according to handling procedures (as described on the next page) to ensure the best cell viability and proper assay performance.

Note: Avoid freezing cells upon receipt as it may result in irreversible damage to the cell line. Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedures.

IMPORTANT: For cells that arrive in a shipping flask please refer to the enclosed ‘cell recovery procedure’.

Cell Line Stability

Cells will undergo genotypic changes over time resulting in reduced responsiveness in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably transfected cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages.

Quality Control

? The overexpression of the human ACE2 (hACE2) gene has been verified by RT-qPCR, FACS staining, and functional assays.

? The overexpression of the human TMPRSS2 (hTMPRSS2) gene has been verified by RT-qPCR and functional assays.

? The stability for 20 passages following thawing has been verified.

? These cells are guaranteed mycoplasma-free.