Contents and Storage

? 3-7 x 106 A549-ASC-NLRP1 cells in a cryovial or shipping flask IMPORTANT: If cells provided in a cryovial are not frozen upon arrival,  contact InvivoGen immediately.

? 1 ml of Blasticidin(10 mg/ml). Store at 4°C or at -20°C.*

? 1 ml of Normocin™ (50 mg/ml), a formulation of three antibiotics active against mycoplasmas, bacteria and fungi. Store at -20°C.*

*The expiry date is specified on the product label. Handling Frozen Cells Upon Arrival Cells must be thawed immediately upon receipt and grown according  to handling procedures (as described on the next page) to ensure the  best cell viability and proper assay performance. Note: Avoid freezing cells upon receipt as it may result in irreversible  damage to the cell line.

Disclaimer: We cannot guarantee cell viability if the cells are not thawed  immediately upon receipt and grown according to handling procedures. IMPORTANT: For cells that arrive in a shipping flask please refer to the  enclosed ‘cell recovery procedure’.

Cell Line Stability

Cells will undergo genotypic changes resulting in reduced  responsiveness over time in normal cell culture conditions. Genetic  instability is a biological phenomenon that occurs in all stably genetically  engineered cells. Therefore, it is critical to prepare an adequate number  of frozen stocks at early passages. To ensure maximum efficiency, do  not passage A549-ASC-NLRP1 cells more than 20 times.

Quality Control

? The expression and functionality of ASC::GFP and NLRP1 has  been verified by Western blot, flow cytometry and fluorescence  microscopy, respectively.

? The stability of this cell line for 20 passages following thawing has  been verified.

? A549-ASC-NLRP1 cells are guaranteed mycoplasma-free

Applications:

• Screening of NLRP1 inflammasome activating stimuli

• Real-time monitoring of ASC-dependent inflammasome assembly

• Rapid and visual screening of drugs targeting the NLRP1 inflammasome by fluorescence microscopy