NEBNext ARTIC kits include primers and reagents for RT-PCR from SARS-CoV-2 gRNA and downstream library preparation for Illumina® and Oxford Nanopore Technologies® sequencing. The V3 ARTIC primers have been balanced, using methodology developed at NEB based on empirical data from sequencing. In combination with optimized reagents for RT-PCR, the kits deliver improved uniformity of amplicon yields from gRNA across a wide copy number range.

If shorter inserts are preferred, the NEBNext ARTIC SARS-CoV-2 FS Library Prep Kit (Illumina) (NEB #E7658) generates libraries with ~150 bp inserts, compatible with 2 x 75 bp sequencing.
·Information on primer variant overlaps can be found here.
·Streamlined, high-efficiency protocol.
·Effective with a wide range of viral genome inputs (10-10,000 copies).
·Improved uniformity of SARS-CoV-2 genome coverage depth with a more balanced primer pool.
·No requirement for amplicon normalization prior to library preparation.
·Library inserts in the 400 bp range.
·Compatible with 2 x 250 Illumina sequencing.
·Includes NEBNext Sample Purification Beads (SPRIselect®).
·Adaptors and primers available separately; For a 384 unique dual index format, please contact us.
·For a bulk format of the kit (2,304 reactions) please contact us.