The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis.

This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.

Features:
·Efficient, specific depletion of rRNA and globin mRNA (adult, fetal and embryonic).
·Suitable for low-quality or high-quality RNA.
·Compatible with a broad range of input amounts: 10 ng - 1 μg.
·Optional integration with poly(A) mRNA isolation workflows for removal of globin RNAs, rRNAs, and noncoding RNAs.
·Fast workflow: 2 hours, with less than 10 minutes hands-on time.