The purified total RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, Northern blotting, RNA sequencing, RNA Chip, and so on.
Exosomes can be derived from ultracentrifugation or isolated by exosome isolation kit, such as Exosome Isolation Kit (from cell culture medium, Cat. No.: EZB-exo2).
Exosome samples are first lysed in a strong denaturant and phenol containing buffer, which immediately inactivates RNases to ensure isolation of intact total RNA. After sample lysis, chloroform is mixed with the lysate and incubated for 3 minutes. And then the mixture is centrifuged for phase separation; the upper-layer supernatant is collected and mixed with ethanol. The liquid is then passed through the RNA binding Spin Column containing a silica-based membrane to which the RNA binds. Impurities are effectively removed by subsequent washing. The purified total RNA is then eluted with Elution Buffer and may be used in a variety of downstream applications.

And the isolated exosomes can be used for RNA purification and detection, such as RT-qPCR, RNA sequencing, RNA Chip, and so on.