The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc. HIGHLIGHTS: ·Easy Handling: Bypass chloroform, phase separation and precipitation steps.
·NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
·Non-Biased: Complete RNA recovery without miRNA loss.