The Neuroporter™ Transfection Kit was designed for difficult-to-transfect primary neurons, addressing past problems such as poor cell viability, low transfection efficiency and neuro-degeneration.
Application:
Suitable for transient and stable transfection of nucleic acids into primary neurons and cultured neuronal cell lines. Use approximately 15-120 μl Neuroporter™ Transfection Reagent and 6-8 μg DNA (in provided unique DNA Dilution buffer when required) per 6 cm cell culture plate. The following cells have been successfully transfected using the Neuroporter™ Transfection Kit:
·C6 glioma (human)..
·Cortical neurons (rat primary).
·Dorsal Root Ganglion (DRG) cells (rat).
·NT2 neurons(human precursor cells).
·NT neurons (human differentiated cells).
·Subventricular Zone (SVZ) cells (mouse).
·White matter cells (mouse).
Features and Benefits:
·Optimized for primary neurons, glial cells, and cultured neural cell lines.
·Very low toxicity with no neuro-degeneration or dendrite withdrawal.
·Efficient DNA delivery primary neurons, glial cells, and cultured neural cell lines.
·Fast and easy to use compared to other methods.
·Compatible with both serum and serum-free transfection protocols.