The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 μl of the supplied phage.
·Ready to use complex phage library.
·Applications and methodologies are numerous and supported by over 20 years of literature citations.
·Does not require helper phage for amplification.
·Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube.