A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
? Defined system with all his-tagged proteins for coupled transcription/translation; Ribosome is not his-tagged.
? T7 RNA Polymerase drives in vitro transcription.
? Minimal nuclease and protease activity for stability of synthesized protein and encoding target.
? Templates can be either plasmid DNA, linear DNA or mRNA.
? Protein of interest can be synthesized and visualized in a few hours.
? Synthesizes various target peptides and proteins.
? Synthesized protein can be co-translationally radiolabeled or fluorescently labeled.
? Protein can be reverse-purified or subject to direct functional analysis.
? Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions.
? Due to reconstituted nature, several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies (see companion products).
? Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820).