The Q5® Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours.
·Non-overlapping primer design ensures robust, exponential amplification, generating a high percentage of desired mutations from a wide range of templates.
·Intramolecular ligation and transformation into NEB high-efficiency competent cells results in high colony yield.
·Extremely low error rate of Q5 Hot Start High-Fidelity DNA Polymerase reduces screening time.
·Hot start polymerase enables room temperature reaction set up.
·DpnI background reduction permits a wide range of starting template concentrations.
·Use of standard primers eliminates additional expenses from phosphorylated or purified oligos.
·Easy-to-use PCR master mix and unique multi-enzyme KLD Mix offer convenience and quality.
·Rapid and direct treatment step proceeds at room temperature in 5 minutes.
·Allows the use of any chemically-competent E. coli cells suitable for cloning.
·Supplied with competent cells.
·Use NEBaseChanger™ tool to generate primer sequences and an annealing temperature.