Strand-specific/directional methods for sequencing RNA provide information on the DNA strand from which the RNA strand was transcribed. This is useful for many reasons including: Identification of antisense transcripts, determination of the transcribed strand of noncoding RNA, and measurement of expression levels of coding or noncoding overlapping transcripts. Overall, the ability to determine the originating strand can substantially enhance the value of a RNA-seq experiment.

The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application.

Features:
·Get more of what you need, with the highest library yields.
·Generate high quality libraries even when you have only limited amounts of input RNA:
·10 ng – 1 μg Total RNA (polyA mRNA workflow).
·5 ng – 1 μg Total RNA (rRNA depletion workflow).
·Minimize bias, with fewer PCR cycles required..
·Increase the complexity and transcript coverage of your libraries
·Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility.
·Rely on robust performance, even with low quality RNA, including FFPE.
·Compatible with NEBNext poly(A) mRNA Isolation, rRNA Depletion reagents and multiplexing adaptors and primers.