This kit can be used with cultured cells and part of animal tissues. Biological samples are first lysed and homogenized in a strong denaturant containing buffer, which immediately inactivates RNases to ensure isolation of intact RNA. After homogenization, the lysate is passed through the DNA Removing Spin Column which traps the DNA on the column, while RNA can pass through the column with the flow-through. Then ethanol is added to the flow-through. The mixture is then passed through the RNA binding Spin Column to which the RNA binds. Impurities are effectively removed by subsequent DNase treatment and washing. Finally, high-quality RNA is eluted in Elution Buffer. The purified RNA is suitable for use in a variety of downstream applications, including: RT-PCR, RT-qPCR, and so on.