The EZBioscience® Cell Viability Detection Kit (Luminescence) provides a homogeneous method to detect cell viability or the number of viable cells in culture by quantitating the amount of ATP present, which indicates the presence of metabolically active cells. This ready-to-use reagent is based on the luciferase reaction, containing thermostable luciferase (luciferase) and high-purity luciferin (luciferin). The homogeneous assay procedure involves addition of ATP Detection Reagent directly to cells cultured in serum-supplemented medium to lyse the cells to release ATP without washing the cells and removal of medium. and the reaction shown in the figure below can occur. The reagent has excellent performance, good linear correlation, high specificity, and long signal half-life. At the same time, the ATP Detection Reagent is much more stable and will maintain > 85% light output upon storage at 4°C for several weeks.