The kit has been optimized for use with S. cerevisiae and C. albicans but can be used for any fungal species that is susceptible to yeast lytic enzyme digestion. The digestion procedure effectively generates spheroplasts of yeast cells, making them ideal for both protein and DNA analyses including Western blotting and PCR, respectively.
HIGHLIGHTS:
·Convenient: Rapid method for efficient lysis of yeast for downstream protein and DNA analyses.
·Versatile: Procedure suitable for any fungal species susceptible to Zymolyase.
·Effective Spheroplasting: Ideal protocol for Western blotting and PCR.