The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
HIGHLIGHTS:
·The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
·100% automation ready with only a single PCR step and without the need for normalization.
·Real-time PCR enables absolute microbial copy number quantification.