Zymo-Seq WGBS Library Kit incorporates tagmentation technology to eliminate the tedious fragmentation, enzymatic, and clean-up steps required by conventional, ligation-based library preparation methods. This streamlined workflow minimizes hands-on time, making it an ideal choice for higher throughput applications. To prepare Zymo-Seq WGBS libraries, intact genomic DNA is first bisulfite converted. Then, the following library preparation procedures are completed in a single tube: (1) second-strand synthesis, (2) adapterization via tagmentation, and (3) library amplification and indexing. After purification, libraries are ready for sequencing on Illumina instruments.
HIGHLIGHTS:
·Whole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube.
·Reduced library preparation bias for accurate methylation calling.
·Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples).