The procedure combines a unique, single-step agarose dissolving/RNA binding buffer with Zymo-Spin column technology to yield high quality, purified RNA in just minutes. The purified RNA is eluted into small volumes of DNase/RNase-free water for highly concentrated samples suitable for subsequent RNA-based manipulations. Compatible with MOPS, TAE, and TBE buffered agarose gels (formaldehyde up to 2.0%).
HIGHLIGHTS:
·Quick and Reliable: 30 minute recovery of purified RNA fragments from agarose gels.
·Concentrated: Up to 10 μg sample in ≥ 6 μl elution.
·High Recovery: ≥ 80% recovery for RNA > 500 nt.