Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Gigaprep kit can process up to 2.5 L of culture in less than 45 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with a vacuum instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 μg/μl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Gigaprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. HIGHLIGHTS:
·Perform plasmid gigapreps in only 45 minutes using a simple spin-column protocol.
·Purify up to 25 mg of highly concentrated plasmid DNA directly from a spin-column.·
·Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.