Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Maxiprep kit can process up to 150 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 μg/μl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Maxiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. HIGHLIGHTS:
·Perform plasmid maxipreps in only 16 minutes using a simple spin-column protocol.
·Purify up to 3 mg of highly concentrated plasmid DNA directly from a spin-column.
·Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.