Reagent :FectoVIR®-AAV.

Molecule delivered :Plasmid DNA and co-delivery of several plasmid DNA.

Applications :
Recombinant AAV virus production in suspension cells.

Virus types :Adeno-Associated Viruses (AAV).

Cell types :Mammalian producer cell lines (HEK-293, HEK-293 derivatives) grown in suspension cultures.

Number of transfections :100 mL of FectoVIR®-AAV transfection reagent is sufficient to transfect on average 50 L of cell culture.

Storage :
Store FectoVIR®-AAV at 5 °C ± 3°C.
Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Provided with:
Protocol.
Certificate of Analysis.
Certificate of Origin.
Non-Hazardous Product Statement.

Summary:
AAVs are becoming the leading viral vector in the development of gene-based advanced therapy medicinal products (ATMPs). Their success is due to their intrinsic properties: they are primarily non-integrating as they persist within cells as episomes, and have a broad tropism with eleven serotypes that have the ability to infect specific cell types. A major challenge today is manufacturing sufficient quantities of AAV in order to treat larger groups of patients. FectoVIR®-AAV is a novel class of animal free transfection reagent specifically developed for industrial scale production of recombinant AAV (rAAV) viral vectors in suspension HEK-293 cell types. FectoVIR®-AAV transfection reagent guarantees higher rAAV viral titers, improved flexibility and scalability for industrial scale manufacturing.