form:fine powder.
technique(s):cell culture | plant: suitable.
application(s):agriculture.
General description:Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Application:Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
as a component of liquid media for the tissue culture of Brassica rapa
as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
as a component of solid GM medium for germination of Arabidopsis seeds