Product Introduction：The His-Spin Protein Miniprep provides a fast spin-column based purification technology for His-tagged proteins. Up to 1 mg of His-tagged protein can be purified in 5 minutes and eluted in as little as 100 μl of His-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

Highlights：

  ●  Fast: Purify His-tagged proteins from cell lysates in less than 5 minutes.

  ●  Simple: Prepare pure protein for small-scale studies using a spin-column.

  ●  Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

Affinity Matrix：Nickel charged agarose

Binding Capacity：1 mg

Elution Method：Imidazole gradient

Elution Volume：100 - 200 μl (150 μl is optimal)

Principle of Technology：Based on a nickel-charged His-Affinity Gel (IMAC).

Processing Time：5 minutes

Product Storage：Please store the His-Affinity Gel, His-Binding Buffer, His-Wash Buffer, and His-Elution Buffer at 4°C. The other components can be stored at room temperature.

Protein Purity：Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.

Required Equipment：Microcentrifuge

Sample Type：Cell lysates or other complex protein mixtures containing His-tagged proteins.