线性聚乙烯亚胺转染试剂的应用

PEI又称为线性化聚乙烯亚胺，是人工合成的含有极高密度正电荷的有机大分子阳离子聚合物。PEI与DNA通过正负电荷的相互吸引形成复合物，这些复合物很容易通过内吞作用进入细胞。PEI-DNA复合物能很好地抵御溶酶体的降解作用，有效提高转染效率。PEI可作用于大至135000个核苷酸的质粒，所以较大的质粒也可以成功转染。目前市面上PEI转染试剂有很多种，Polysciences的PEI线性转染试剂适用于多种真核细胞，用途广泛。

一、特性：

货号：23966-1

分子式：(CH₂CH₂NH)_n

分子量：25000

外观：白色至黄色粉末

熔点：73-75℃

溶解性：溶于：热水、低pH值的冷水、甲醇和乙醇；

不溶于：苯、乙醚和丙酮。

二、Polysciences聚乙烯亚胺转染试剂（PEI 25K）的应用：

递送物质	转染细胞类型	转染细胞举例（包括但不限于）
DNA	贴壁/悬浮细胞	HEK293
		CHO
		HELA
		SF9昆虫细胞
		SF21昆虫细胞等真和细胞系

三、应用Polysciences PEI线性转染试剂发表的高分文章：

(1)Y. Durocher, et al. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research, 2022, 30(2), e9-e9.  IF：19.160

试剂：PEI 25K；应用：DNA转染人胚肾293S细胞。

(2)S. Siegman, et al. Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta biomaterialia, 2015, 28, 45-54.  IF：10.633

试剂：PEI 25K；应用：质粒DNA转染小鼠骨髓间充质干细胞（D1）。

(3)M. P. Trefny, et al. PD-1+ natural killer cells in human non-small cell lung cancer can be activated by PD-1/PD-L1 blockade. Cancer Immunology, Immunotherapy: CII., 2020.  IF：6.630

试剂：PEI 25K；应用：包装质粒pCMVdelta8.9、包膜质粒VSV-G和PD-1-GFP、PD-L1-GFP或空GFP sFFV转移质粒转染HEK293T细胞

(4)C. Schmitz, et al. Live reporting for hypoxia: Hypoxia sensor–modified mesenchymal stem cells as in vitro reporters. Biotechnology and Bioengineering, 2020, 117(11), 3265-3276.  IF：4.395

试剂：PEI 25K；应用：转染HEK293T细胞。

(5)J. Li, et al. Targeting eIF3f Suppresses the Growth of Prostate Cancer Cells by Inhibiting Akt Signaling. OncoTargets and therapy, 2022, 13, 3739.  IF:4.345

试剂：PEI 25K(23966-1)；应用：质粒转染HEK293FT细胞。

(62)S. Bang, et al. The Clinical Relevance and Function of Krüppel-Like Factor 16 in Breast Cancer. Cancer Management and Research, 2020, 12, 6373.  IF：3.602

试剂：PEI 25K(23966-1)；应用：用shRNA-PLKO.1质粒转染HEK293FT细胞。

(7)S. Wulhfard, et al. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of biotechnology, 2010, 148(2-3), 128-132. IF：3.595

试剂：PEI 25K；应用：质粒DNA转染悬浮生长的CHO DG44细胞。

(8)A. Sanzhez-Mata, et al. Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs. STAR protocols, 2020, 1(3), 100183.

试剂：PEI 25K；应用：用DNA转染HEK293T细胞。

(9)M. Thomas, et al. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proceedings of the National Academy of Sciences, 2005, 102(16), 5679-5684.

试剂：PEI 25K；应用：将编码fLuc基因的DNA递送至小鼠。

(10)L. Baranyi, et al. Rapid generation of stable cell lines expressing high levels of erythropoietin, factor VIII, and an antihuman CD20 antibody using lentiviral vectors. Human Gene Therapy Methods, 2013, 24(4), 214-227.

试剂：PEI MAX(24765)；应用：用质粒DNA转染ET801载体—幼仓鼠肾来源细胞。

北京百奥创新科技有限公司提供多款Polysciences转染试剂，具体信息请咨询百奥创新客服。(现货促销，惊爆价！机会有限，不要错过！)